Table 1.
Percent content of variously substituted glucosamine and uronic acid residues in different disaccharide sequences of Fragmin® samples, together with typical linkage region residues.
| Frag-1 | Frag-2 | Frag-3 | Frag-4 | Frag-5 | Frag-6 | Frag-7 | Frag-8 | Frag-9 | Frag-10 | Frag-11 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Amines | |||||||||||
| ANS-(I2S) | 55.7 | 56.6 | 55.2 | 55.8 | 57.2 | 58.1 | 56.3 | 57.2 | 59.0 | 55.2 | 55.7 |
| ANS-I | 7.6 | 5.9 | 7.0 | 7.6 | 6.6 | 6.8 | 6.9 | 7.1 | 6.5 | 7.5 | 8.1 |
| ANS-(G) | 5.9 | 6.0 | 5.8 | 5.9 | 5.9 | 5.6 | 6.0 | 5.9 | 5.7 | 5.6 | 5.6 |
| A* | 5.9 | 5.5 | 6.1 | 5.3 | 5.6 | 4.9 | 5.5 | 5.2 | 4.7 | 5.5 | 5.1 |
| ANAc-(G) | 9.4 | 9.5 | 9.0 | 9.5 | 9.3 | 9.2 | 9.2 | 9.3 | 8.9 | 9.7 | 9.7 |
| ANAc-(I) | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd |
| ANH2 | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd |
| A-epox | nd | nd | 0.8 * | 0.6 * | 0.6 * | 0.7 * | 0.8 * | 0.6 * | nd | 1.1 * | nd |
| A-(GalA) | 1.6 | 1.9 | 1.4 * | 1.0 * | 1.1 * | 1.1 * | 0.5 * | 1.0 * | 0.9 * | 2.0 | 1.4 |
| A6S | 90.7 | 90.5 | 90.6 | 90.4 | 89.9 | 90.3 | 90.6 | 89.8 | 90.2 | 91.0 | 90.8 |
| aM.ol | 12.9 | 13.3 | 13.5 | 13.3 | 12.9 | 12.6 | 13.9 | 12.7 | 13.2 | 12.7 | 13.3 |
| Rc | 1.0 * | 1.2 * | 1.0 * | 0.9 * | 0.8 * | 1.0 * | 1.0 * | 0.9 * | 1.1 * | 0.7 * | 1.2 * |
| Uronic acids | |||||||||||
| I2S | 75.3 | 77.3 | 76.4 | 76.9 | 76.2 | 76.5 | 75.8 | 76.6 | 76.7 | 73.2 | 74.1 |
| I-(A6S) | 8.3 | 7.2 | 7.4 | 7.2 | 7.0 | 7.4 | 7.1 | 7.0 | 7.0 | 8.3 | 8.6 |
| I-(A6OH) | 0.6 | 0.7 * | 0.8 * | 1.0 * | 0.7 * | 0.7 * | 0.9 * | 0.9 * | 0.7 * | 0.8 * | 0.9 * |
| G-(A*) | 4.9 | 4.4 | 4.4 | 4.4 | 4.4 | 4.5 | 4.7 | 4.4 | 4.5 | 5.0 | 4.7 |
| G-(ANS) | 6.0 | 5.2 | 5.8 | 5.8 | 5.9 | 5.4 | 5.8 | 5.3 | 5.7 | 5.7 | 6.8 |
| G-(ANAc) | 3.9 | 4.6 | 3.9 | 4.1 | 4.5 | 4.7 | 4.9 | 4.5 | 5.3 | 4.1 | 3.8 |
| Gnr | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd |
| epox | nd | nd | 0.8 * | 0.6 * | 0.6 * | 0.7 * | 0.7 * | 0.6 * | nd | 1.0 * | nd |
| GalA | 1.1 | 0.7 * | 0.5* | nd | 0.5 * | nd | nd | 0.7 * | nd | 1.7 | 1.1 * |
| Linkage region | |||||||||||
| Gal1+G | 1.6 * | 1.2 * | 1.6 | 2.1 | 2.0 | 1.8 | 2.1 | 2.0 | 1.9 | 1.3* | 1.4 |
| Gal2 | 1.3 * | 1.0 * | 1.3 * | 1.6 | 1.5 | 1.4 * | 1.6 | 1.4 | 1.6 | 1.2 * | 1.0 * |
| Xyl-Ser-ox | 0.8 * | 0.6 * | 0.8 * | 0.9 * | 0.9 * | 1.0 * | 1.0 * | 0.9 * | 1.1 | 0.6 * | 0.7 * |
| Xyl-Ser | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd |
nd indicates values under the limit of detection (LOD); * indicates values under the limit of quantification (LOQ); Gnr indicates glucuronic acid located at the non-reducing end.