Table 2.
Details of the Taqman® and SYBR® gene expression assays used for real-time polymerase chain reaction (qPCR).
| Gene | Primers Sequence | Dye | Assay ID |
|---|---|---|---|
| Slc2a4 | Inventoried | FAM | Mm01245502_m1 |
| Slc2a2 | Inventoried | FAM | Mm00446229_m1 |
| Gapdh | Inventoried | VIC | Mm99999915_g1 |
| Pck1 | Inventoried | FAM | Mm01247058_m1 |
| Atp5b | Inventoried | FAM | Mm00443967_g1 |
| Tnf | F: GAACTGGCAGAAGAGGCACT | SYBR | |
| R: GGTCTGGGCCATAGAACTGA | |||
| Il6 | F: CCGGAGAGGAGACTTCACAG | SYBR | |
| R: TCCAGTTTGGTAGCATCCATC | |||
| Actb | F: ACTGGGACGACATGGAGAAG | SYBR | |
| R: GGGGTGTTGAAGGTCTCAAA | |||
| Rela | F: GCAAGGGCATTATCGACTCT | SYBR | |
| R: CATAACGTTGCAGGAAGCTG | |||
| Nfkb1 | F: CTGACCTGAGCCTTCTGGAC | SYBR | |
| R: GCAGGCTATTGCTCATCACA |
Assay ID identifies the specific TaqMan® RNA Assay (Applied Biosystems Inc., USA); SYBR® refers to SYBR® Green Real-Time PCR Master Mix (Termo Fisher Scientific). Slc2a4, solute carrier family 2 member 4; Slc2a2, solute carrier family 2 member 2; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; pck1, phosphoenolpyruvate carboxykinase 1; Atp5b, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; Tnf, tumor necrosis factor; Il6, interleukin 6; Actb, actin beta; Rela, RELA proto-oncogene, NF-κB subunit; Nfkb1, nuclear factor kappa B subunit 1.