Figure 6.

The Lineweaver-Burk plots of DL0410, compound 6-1 and compound 7-6 on AChE inhibitory activity (A–C). The Lineweaver-Burk plots of DL0410, compound 6-1 and compound 7-6 on BuChE inhibitory activity (D–F). Kinetic analysis of AChE and BuChE was performed by using three effective concentrations of DL0410, compound 6-1 and compound 7-6 (0.1, 0.3, 1 μM for AChE, and 0.3, 1, 3 μM for BuChE) with the incubation of five different substrate concentrations (1.25, 2.5, 5, 10, 20 mM for AChE, and 2, 4, 8, 16, 32 mM for BuChE). The intersections of fitting lines with different concentrations of the three compounds were neither on the X axis or Y axis of Lineweaver-Burk plots of both AChE and BuChE, suggesting that DL0410, compounds 6-1 and 7-6 were mixed-type of inhibitors. (n = 3; a = mM⁻¹; b = min/ΔA).