Figure 1.

Evodiamine inhibited Mus musculus hepatoma, Hepa1-6 cell proliferation. Mus musculus hepatoma cell line and Hepa1-6 cells were treated with different doses of evodiamine for 24 h (A) or treated with 10 μM evodiamine and harvested at the indicated periods (B). The cells were then incubated with 0.2 mg/mL MTT in culture medium for three hours. The absorbance was measured at a wavelength of 570 nm. The data are expressed as means ± S.E.M. and obtained from two independent experiments, with three replicates for each experiment. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the control group.