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Annexin V-FITC/Propidium iodide (PI) dual staining assay of SMMC-7721 cells treated with compound 4b using flow cytometry (A); Annexin V+/PI− indicated early phase of apoptosis (B); Annexin V+/PI+ indicated late apoptosis (C). *** p value < 0.001 for compound 4b treatment, compared with the DMSO-treated control group.
