Figure 4.

Protective effect of quercetin on H₂O₂ induced apoptosis in PC-12 cells and organotypic hippocampal slice were evaluated by TUNEL assay. (A) Representative images of TUNEL assay in PC-12 cells (400×); (B) quantification of TUNEL-positive cells in A; data collected from 10 fields for each group; experiments were repeated three times; (C) representative images of TUNEL assay in organotypic hippocampal slice (100×); and (D) quantification of TUNEL-positive cells in (C); data collected from 10 fields for each group; experiments were repeated three times. Data are expressed as overall mean ± SD of the ratio of apoptotic cells to the total number of cells. Statistical significance was determined using the Student’s t-test (** p < 0.01vs. control; ^## p < 0.01, ^# p < 0.05 vs. model). Con, untreated; Mod, H₂O₂ treated; Prot, quercetin + H₂O₂ treated.