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. 2017 Jul 21;22(7):1231. doi: 10.3390/molecules22071231

Table 5.

Effect of different temperatures and heat treatment times on AD on tyrosinase activity.

Sample Concentration of Water Extract (Equal to the Amount of Raw Medicinal Herbs)
1 g·mL−1 0.5 g·mL−1 0.25 g·mL−1 0.125 g·mL−1 0.0625 g·mL−1
Crude AD 27.07 ± 0.47 13.42 ± 0.98 9.14 ± 0.83 12.31 ± 1.16 17.29 ± 1.47
50 °C-54 h 20.84 ± 0.65 *** 12.60 ± 0.83 11.85 ± 0.41 13.53 ± 0.90 15.83 ± 0.55
50 °C-108 h 18.43 ± 0.26 *** 11.86 ± 0.68 14.94 ± 1.23 16.00 ± 0.48 4.82 ± 0.40
50 °C-162 h 15.49 ± 0.38 *** 10.09 ± 0.86 14.73 ± 0.66 15.25 ± 0.17 16.80 ± 1.52
50 °C-216 h 14.48 ± 0.46 *** 0.86 ± 0.04 −6.16 ± 0.33 4.91 ± 0.24 10.05 ± 0.22
60 °C-18 h 13.49 ± 0.46 *** 7.97 ± 0.51 4.67 ± 0.18 7.35 ± 0.53 12.44 ± 0.11
60 °C-36 h 9.43 ± 0.42 *** −3.72 ± 0.27 −8.36 ± 0.28 −8.33 ± 0.36 −5.17 ± 0.51
60 °C-54 h 11.50 ± 0.44 *** 5.29 ± 0.48 7.03 ± 0.68 10.88 ± 0.37 15.16 ± 0.42
60 °C-72 h 7.40 ± 0.44 *** −6.21 ± 0.16 −7.38 ± 0.17 −7.32 ± 0.33 7.50 ± 0.43
70 °C-6 h 7.12 ± 0.44 *** −2.82 ± 0.14 −4.33 ± 0.42 −0.28 ± 0.01 4.56 ± 0.32
70 °C-12 h 4.90 ± 0.45 *** −5.34 ± 0.30 −7.46 ± 0.28 −3.86 ± 0.29 −8.56 ± 0.32
70 °C-18 h 0.60 ± 0.06 *** −5.48 ± 0.53 −3.53 ± 0.32 −3.24 ± 0.21 −0.12 ± 0.01
70 °C-24 h 0.55 ± 0.05 *** −5.30 ± 0.28 3.82 ± 0.14 7.53 ± 0.60 5.70 ± 0.30
80 °C-2 h 6.73 ± 0.43 *** −3.26 ± 0.30 −7.23 ± 0.43 −9.05 ± 0.32 −3.10 ± 0.03
80 °C-4 h 8.88 ± 0.50 *** −4.78 ± 0.18 −1.12 ± 0.06 −0.68 ± 0.03 4.47 ± 0.28
80 °C-6 h 11.38 ± 0.44 *** −4.96 ± 0.23 0.74 ± 0.04 1.39 ± 0.10 12.11 ± 0.43
80 °C-8 h 15.43 ± 0.47 *** 5.47 ± 0.18 4.38 ± 0.32 10.81 ± 0.76 2.67 ± 0.08

Note: compare with crude AD, * p < 0.05, ** p ≤ 0.01, *** p ≤ 0.001.