Figure 4.

Comparison of the heteronuclear steady-state {¹H}-¹⁵N nuclear Overhauser effect (NOE) values for Msi1(109–200) in its free and bound forms. The heteronuclear steady-state {¹H}-¹⁵N NOE values are shown for Msi1(109–200) in the free form (black squares) and in the complex form with r(GUAGU) (red circles). The secondary structure elements (helices: yellow cylinders; β-strands: blue arrows) of Msi1(109–200) are shown at the top. The amino acid residues whose ¹H-¹⁵N resonances were not assigned are indicated by black circles (free form) and a red square (complex form) beneath the amino acid sequence at the bottom. Daggers indicate the residues with overlapped ¹H-¹⁵N resonances, while double-daggers indicate the residues whose ¹H-¹⁵N resonance was missing in the free form.