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DOX, Cu-DOX, Zn-DOX, Co-DOX, Ni-DOX, Fe-DOX, Mn-DOX and Mg-DOX cytotoxicity in MCF-7 cells. The cells were incubated with 0.5 µM, 0.1 µM, 0.05 µM and 0.01 µM DOX and Metal-DOX complexes for 24 h. Mean values from five independent experiments ± SD are shown. Significant alterations are expressed relative to control untreated cells (marked with asterisks *) and to DOX—controls (marked with crosses #). Statistical significance was considered if #* p < 0.05.
