Fig. 7.

DLS and TEM particle size analysis from ultra-centrifuged cell-free supernatant. (a) Dynamic light scattering (DLS) measurements of washed ultra-centrifuged particles in Milli Q water. Polydispersity (mean±std. error) values from DLS confirm the size distributions visualized in the TEM data in panel (b). (b) Transmission electron microscopy of the same particles post fixation and dehydration; scale bar, 100 nm. (c) Size comparison of light and dark particles on TEM images as measured with ImageJ. Among the light (less electron-dense) particles, both Uvitex and Eosin Y were significantly smaller than the unstained control.