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Stability of ARG-1 constructs containing ASL or C3 UTRs. Cells were transfected (see Methods Section) with mRNA and incubated for 1 hr. mRNA was removed and cell incubated for indicated time points. mRNA half-life for Arg1 containing C3 (32 min) and ASL (40 min) UTRs was calculated using GraphPad prism. Lines represent Percent change from time 0 (after removal of mRNA from cells) ± 1STDEV.
