Table 1.
UTRs selected for initial assay validation.
| UTR source | Protein type | Liver expression (RPKM) | 5′UTR NT length | 3′ UTR NT length | Kozak sequence |
|---|---|---|---|---|---|
| Ref UTR | N/A | N/A | 47 | 119 | GCCACCATG |
| ORM1 (Orosomucoid 1) | Secreted | 7629.9 | 114 | 129 | CTCAGTATG |
| HPX (Hemoexin) | Secreted | 1326.1 | 64 | 182 | CTCAGCATG |
| FGA (Fibrinogen alpha chain) | Secreted | 4388.0 | 57 | 243 | GAAAAGATG |
| CYP2E12e1 (cytochrome P450 2E1) | Intracellular | 1681.4 | 37 | 161 | GGCACCATG |
| C3 (complement component 3) | Secreted | 1287.5 | 92 | 55 | AGCACCATG |
| ASL (Argininosuccinate lyase) | Intracellular | 87.0 | 239 | 436 | CCCAACATG |
| APOA2 (Apolipoprotein A-II) | Secreted | 3160.8 | 58 | 121 | ACCAACATG |
| ALB (Albumin) | Secreted | 17963.0 | 75 | 355 | GGCACAATG |
| AGXT (Alanine-glyoxylate aminotransferase) | Intracellular | 1086.5 | 121 | 306 | CGGACCATG |
1
Protein type reference to the role of the endogenous ORF from which UTR's were collected.
2
Reads per kilobase million (RPKM) data were obtained from human protein atlas (http://www.proteinatlas.org/).
3
Nucleotide (NT) length based on modified sequences (see Methods section).