Figure 4.
Standard tRNAs do not affect the splicing of their hosting genes. (A) Schematic structure of the At3g12587 hosting tRNA-ArgCCG within its intron. The white box represents an exon, the gray boxes represent UTRs, the lines depict introns, and the arrow corresponds to the tRNA (adapted from TAIR10). (B) Schematic representation of the oligosaccaryltransferase gene variants used. NOS, transcription terminator. (C) Splicing isoforms of the oligosaccaryltransferase transcript recorded by RT-PCR in infiltrated N. benthamiana leaves expressing the oligosaccaryltransferase gene variants shown in B. Schematic structures of the identified isoforms are presented on the left. MOCK is a negative control for the agroinfiltration experiment (leaves infiltrated only with MES buffer). ctrl, amplification of genomic DNA; NTC, non-template control; and marker, 100 bp plus (Thermo Fisher Scientific).