Figure 6.

Activation of the UPR by misreading tRNAs in vivo. A) Activation of ATF6 in tumors harboring the wild-type and misreading tRNAs. Total ATF6 and ATF6 fragment were detected by immunoblotting. B) eIF2α-P and PP1Α catalytic subunit levels in each tumor lysate were analyzed by immunoblotting and relative expression values are shown. β-tubulin levels served as protein loading control. C) Representative immunoblots for total ATF6, ATF6 fragment, total eiF2α, eIF2α-P, PP1α catalytic subunit and β-tubulin for each membrane. Graphics depict average ± SEM (n = 3). Data was analyzed by One-way ANOVA with Dunnett's post-test and significant p-values are shown (*p < 0.05).