Figure 1.
The expression of thiM is modulated through Rho-dependent transcription termination. RNase H cleavage assays show that a region (black) is protected in the presence of Rho, consistent with the presence of a rut. Rho-dependent transcription termination sites identified in vitro are shown in gray and codons (cd) 9, 20, 34 and 40 are underlined.