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. 2018 Jul 30;10(6):922–933. doi: 10.1080/19420862.2018.1486355

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© 2018 The Author(s). Published with license by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — SDS-PAGE analysis of NISTmAb RM 8671, NS0-59, NS0-60, and NS0-66.

SDS-PAGE analysis was used to evaluate the RM 8671, NS0-59, NS0-60 and NS0-66 antibodies in the presence (lanes 3, 5, 7, and 9) or absence (lanes 2, 4, 6, and 8) of 700 mmol/L BME. Protein samples (1.25 μg) were separated on a 4–20% gradient SDS-PAGE followed by staining with GelCode Blue Safe Protein Stain. Lane 1, molecular weight marker (kDa); lanes 2 and 3, RM 8671; lanes 4 and 5, NS0-59; lanes 6 and 7, NS0-60; lanes 8 and 9, NS0-66. mAb, full length antibody; HC, heavy chain; LC, light chain.