Figure 1. Promotion of K-ras induced lung tumorigenesis by inflammation is associated with increased angiogenesis and activation of the HIF-1α pathway.

(A) Representative photomicrographs of CD31 immunolabeled lung tumor tissues of 14 weeks old CC-LR mice in the absence or presence of NTHi-induced COPD-type airway inflammation (40× magnification, scale bar = 100 μm, applicable to all panels). (B) Quantitative analysis of CD31 positive staining in lung tissue of CC-LR mice in the absence or presence of NTHi exposure (mean ± SE; ^* = P ≤ 0.05 for CC-LR vs CC-LR plus NTHi; n = 3). (C) Western blot analysis of HIF-1α in the whole lung tissue nuclear extract. (D) Western blot analysis of VEGF in protein extracted from whole lung tissue.