Figure 4. Structure and effect of the HIF-1α transgene expression in the airway epithelium.

(A) Schematic representation of HIF-1α transgene construct and transgenic mouse development. (B) Immunoblotting to confirm endogenous and transgenic HIF-1α protein production. (C, D) Representative photomicrographs of H&E stained lungs from HIF-1α transgene negative and positive mice showing airspace enlargement and alveolar wall destruction after doxycycline treatment in transgene positive but not negative mouse.