Figure 3.

In vitro synergistic effects of flavopiridol and carfilzomib in monolayer culture (A), and in three-dimensional multicellular aggregates (B) of NCI-H295R and SW13 cells. (C) In vitro increased G2M cell cycle arrest (left panels) and apoptosis (right panels) in ACC cells treated with the combination of flavopiridol and carfilzomib. Drugs are represented by the initial letters or abbreviated names followed by the concentrations in nM. For example, F100 or Flavo 100 = flavopiridol 100 nM, C10 or Carf 120 = carfilzomib 10 nM or carfilzomib 120 nM, respectively, F50/C10 = the combination of flavopiridol 50 nM and carfilzomib 10 nM, and Ctrl = vehicle control. Duration of treatment is presented in days (D1-D6).