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. 2018 Aug 31;9(68):32943–32957. doi: 10.18632/oncotarget.25993

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Copyright: © 2018 Chung et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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To verify the anti-inflammatory effects of resveratrol, HCT116 and DLD1 were treated with resveratrol (100 μM) over the time course of 0, 6, 12 and 24 hours. (A) In HCT116, inflammation biomarkers pSTAT3 Y705, pSTAT3 S727 and pNFkB S were monitored over the resveratrol treatment on a time course. (B) DLD1 was treated with resveratrol (100 μM) over 24 hours and examined for inflammation biomarker status. Next, colorectal cancer cells were treated with either 5-FU or resveratrol alone or combined, then the key proteins were analyzed by western blotting. Densiometric values of protein bands were corrected based on β-actin and were expresses relative to that of untreated control cell, which was set as 1.0.