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. 2018 Aug 16;14(11):1943–1958. doi: 10.1080/15548627.2018.1493043

Figure 8.

Figure 8.

Trehalose but not rapamycin promoted PARK7 secretion in WT MEF cells. (A) WT MEF cells were treated with 100 nM BafA1 for 1 h and were then treated with 0–2 μM rapamycin for 3 h. Whole cell lysates were immunoblotted using antibodies specific for LC3B or ACTB. LC3B and ACTB band intensities were quantified by densitometric scanning and LC3B-II:ACTB ratio is shown. n = 3; mean ± S.D.; **, p < 0.01. (B) WT MEF cells were treated with 1–5 μM rapamycin or 75 μM 6-OHDA for 3 h and were then cultured in serum-free medium for 2 h. Whole cell lysates and the conditioned medium were immunoblotted using antibodies specific for PARK7 or ACTB. PARK7 band intensities were quantified by densitometric scanning and relative secretion level to vehicle-treated cells is shown. n = 3; mean ± S.D.; **, p < 0.01. (C) WT MEF cells were treated with 0–200 mM trehalose for 48 h. Whole cell lysates were immunoblotted using antibodies specific for LC3B or ACTB. LC3B and ACTB band intensities were quantified by densitometric scanning and LC3B-II:ACTB ratio is shown. n = 3; mean ± S.D.; *, p < 0.05; **, p < 0.01. (D) WT MEF cells were treated with 200 mM trehalose for 48 h or 75 μM 6-OHDA for 3 h and were then cultured in serum-free medium for 2 h. Whole cell lysates and the conditioned medium were immunoblotted using antibodies specific for PARK7 or ACTB. PARK7 band intensities were quantified by densitometric scanning and relative secretion level to vehicle-treated cells is shown. n = 3; mean ± S.D.; *, p < 0.05.