Figure 3.

SQSTM1/p62 directly interacts with and regulates the levels of USP14. (a) Schematic of SQSTM1 mutant constructs. (b) 293T cells were transfected with the indicated constructs, followed by immunoprecipitation as indicated and immunoblotting using the anti-GFP and -HA antibodies. The corresponding whole cell lysates (WCL) were used as input controls and probed with the indicated antibodies. (c and d) Representative confocal images and quantification of SQSTM1 and USP14 levels and colocalization in C4-2 cells stably expressing shCtrl or shATG7 at the indicated time following IR. Nuclei were stained with DAPI. Data shown are the means ± SEM (n  =  2); P < 0.0001****.