Figure 4.

miRNA Biogenesis Pathway is Altered during Adipogenic and Osteogenic Differentiation of Adipose Derived Stem Cells. ASCs were grown in either stromal, osteogenic, or adipogenic media and were collected for RNA extraction at different intervals up to 2 weeks. qPCR was performed to evaluate expression of genes associated miRNA stability (A and C) or cleavage (B and D). Error bars represent SEM, n = 3, normalization was to stromal media, and Cycb gene expression. Fold change represents relative gene expression changes. * Significantly different p<0.05.