Figure 1.

Inhibition of FGFR1 attenuates HuFTF-stimulated transformation of MCF-10A cells. HuFTF significantly stimulated colony formation above the untreated control. Cells were treated with 200 μg/mL of HuFTF protein. HuFTF-stimulated growth in soft agar was significantly attenuated by the FGFR1 Ab. MCF-10A ells were treated with a FGFR1 neutralizing antibody (FGFR1 Ab) at 2 μg/mL and treated with HuFTF from six different donors. The percent of colony formation was calculated as described in Methods, MCF-10A cells were cultured as described in Methods. Data is presented as mean ± six biological replicates. Each biological replicate had three technical replicates. Confidence intervals (CI) were calculated for HuFTF treated MCF-10A cells (95% CI 3.024–7.658) and for HuFTF+Ab (95% CI 0.093–2.929). Statistical significance between HuFTF and control and HuFTF and HuFTF+FGFR1 Ab was determined by unpaired t-test (*p < 0.05).