Figure 3.

FGF2-tranformed MCF-10A cells are morphologically and functionally distinct from parent MCF-10A cells. (A) MCF-10A cells have epithelial-like morphology and transformed MCF-10A cells have spindle-like morphology. Transformed MCF-10A cells were obtained by treating MCF-10A cells with FGF2 in soft agar. After 14 days, a colony was isolated and cultured in traditional cell culture conditions for several passages, making transformed MCF-10A cells. Untreated transformed MCF-10A cells formed over 44% more colonies in soft agar compared to untreated parent MCF-10A cells. The percent of colony formation was calculated ([colonies counted × 100] 750 cells).