Figure 5.
Expression of autophagy proteins in response to CCA in C2C12 myotubes treated with bafilomycin A1 (BafA). Representative western blots. (A) Whole cell extracts were probed for SQSTM1 and GAPDH, and (C) mitochondrial fractions were probed for SQSTM1 and VDAC. Graphical densitometric quantification. (B) Total SQSTM1 normalized to GAPDH (†P < 0.01, main effect of BafA vs. vehicle; * P < 0.05, vehicle BafA vs. BafA + CCA; #, P < 0.05 interaction effect between CCA and BafA treatment, n = 5). (D) Mitochondrial SQSTM1 (†P < 0.001, main effect of BafA vs. vehicle; *P < 0.01, BafA control vs. BafA + CCA; n = 3). A.U., arbitrary units.