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. 2018 Aug 10;14(11):1991–2006. doi: 10.1080/15548627.2018.1501132

Figure 7.

Figure 7.

Atg7cKO:apoeKO mice under Western diet shows increased apoptotic cell death in plaque and media. (A) Representative histological images of abdominal aorta stained with SQSTM1 antibody from control Atg7f/f:apoeKO mice (Control) and Atg7cKO:apoeKO mice (cKO:KO) fed a Western diet. Scale bars: 50 μm. (B) Transmission electron microscopy (TEM) images of abdominal aorta from Control and cKO:KO under Western diet. The asterisks indicate cytosolic inclusions. The white arrowheads indicate SMC nuclei with normal morphology. The boxed areas indicate dying SMCs of cKO:KO with pyknotic nuclei, and their enlarged images are shown in the insets. The white arrow indicates inflammatory cell infiltration into the media. Scale bars: 1 μm in the upper panel, and 50 μm in the lower panel. (C) Representative images of the TUNEL assay and Red Counterstain C staining of the abdominal aorta of Control and cKO:KO fed a Western diet. Scale bars: 50 μm. (D) The area of TUNEL-positive lesions in the media and plaques of the abdominal aorta was evaluated. Data are mean ± SEM of 11 Control and 21 cKO:KO each. *P < 0.05 vs Control.