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. 2018 Aug 6;14(11):1959–1975. doi: 10.1080/15548627.2018.1493044

Figure 4.

Figure 4.

Trehalose induces hepatocyte FGF21 expression. (A) Fgf21 mRNA accumulation in mice treated with trehalose for 24 h with 3% trehalose water ad libitum (n = 5 mice per group). (B) Hepatic FGF21 protein in livers of mice treated with or without 3% trehalose water ad libitum (n = 5 mice per group). ACTB is used as a loading control. (C) FGF21 ELISA showing circulating FGF21 levels in wild-type mice in response to sterile water or 3% trehalose water. (D) Fgf21 mRNA accumulation in primary hepatocytes (left) and AML12 mouse hepatocytes (right) treated with or without trehalose for 24 h. (E) FGF21 immunoblot showing increased FGF21 peptide in primary hepatocytes treated with or without trehalose from 8–24 h. (F) Fgf21 mRNA accumulation in primary hepatocytes treated with or without trehalose in the presence or absence of 5 mM pyruvate. * P < 0.05, *** P < 0.001, **** P < 0.0001 versus control by Student’s T-test with Bonferroni-Dunn post-hoc correction for multiple tests.