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. 2017 Jun 7;22(6):949. doi: 10.3390/molecules22060949

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Viability of RAW 264.7 cells; (B) Nitric oxide content of RAW 264.7 cell supernatant and (C) mRNA expression of pro-inflammatory cytokines (tumor necrosis factor (TNF-α) and IL-1β) relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (∆∆C_t) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells in the presence of intact β-lactoglobulin (BLG) and BLG hydrolysates produced by alcalase under HHP and AP conditions (BLG-HHP-Alc and BLG-AP-Alc). The Negative Control indicates the cells grown without any treatment. The Positive Control indicates the cells treated with LPS, without treatment. The bars and error bars represent means and standard errors, respectively (n ≥ 3). Means with different lowercase letters differ significantly (p < 0.05).