Table 1.

The enzymes applied in high hydrostatic pressure combined with enzymatic hydrolysis (HHP–EH) and atmospheric hydrolysis, and the operational conditions.

Enzyme	Sources	Proteolytic Activity a	Optimum Conditions
Neutrase	Protease from Bacillus amyloliquefaciens	≥0.8 U/g	pH 7; 37 °C
Alcalase	Protease from Bacillus licheniformis	≥2.4 U/g	pH 7; 50 °C
Savinase	Protease from Bacillus sp.	≥16 U/g	pH 7; 55 °C
Elastase	Elastase from hog pancreas	≥4 U/mg	pH 8; 37 °C
Thermolysin	Protease from Bacillus thermoproteolyticus	14 U/mg	pH 7; 50 °C
Trypsin	Protease derived from porcine pancreas	30 U/g	pH 7; 37 °C

^a Minimum proteolytic activity of the enzyme at optimum pH and temperature.