Figure 5.

Effects of Baccharis trimera aqueous extract (AE) and AE with decoction (AE-D), both with adipogenic growth medium (MDI), on the expression of adipocyte markers. (A) Equal amounts of protein (50 µg) of cell lysate were analyzed by Western blotting to detect β-actin, CCAAT enhancer-binding protein α (C/EBPα), CCAAT enhancer-binding protein β (C/EBPβ), and gamma receptors by peroxisome proliferators (PPARγ) levels; (B) The graph of the protein relative expressions was obtained as described in materials and methods. Control, Control with MDI. Values are expressed as mean ± standard deviation (n = 3). Letters a,b,c,d indicate significant differences between different concentrations and the control. Numbers 1,2,3,4,5 indicate significant differences between each protein analyzed in the different concentrations between each extract as calculated by one-way analysis of variance (ANOVA) followed by the Student’s t-test (p < 0.05).