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Chromatogram from a high performance liquid chromatography with diode array detector (HPLC-DAD) trial of the aqueous extract with decoction (AE-D) of Baccharis trimera: (A) Method 1; and (B) Method 2. SP: column C18 Phenomenex-Luna® (250 × 4.6 mm, 5 µm); Mobile phase: acetonitrile gradient: acetic acid 0.3%; Detection: 340 nm. Chlorogenic acid was identified (arrow).
