Table 2.
Comparison of relevant properties concerning hydrophilicity of a series of structurally similar synthetic bacteriochlorins, as described by the group of Lindsey [65].
| Compounds | Purification Method a | Water-Solubility b | FWHM (abs, fl) (nm) c | Stability in Light (%) d | Stability in Dark (%) e |
|---|---|---|---|---|---|
| 132 | reverse phase chromatography | 0.46 μM–460 μM | 26, 30 | 85 | >95 |
| 92 | ppt, washing with hexanes/MeOH (49:1) | 0.32 μM–320 μM | 26, 26 | 96 | >95 |
| 22 | ppt, washing with Et2O/THF (1:1) | 0.24 μM–240 μM | 26, 26 | 82 | >95 |
| 23 | ppt.,washing with Et2O/THF (1:1) | 0.18 μM–180 μM | 40, 26 | 96 | >95 |
| 176 | ppt, washing with hexanes/CH2Cl2 (19:1) | 0.43 μM–430 μM | 22, 23 | 95 | >95 |
a For the final step of the synthesis; b measured in aqueous media (PBs except 176, water) by the absorption upon reciprocal change in concentration and path length; c 1 μM in PBS. FWHM = full width at half maximum of the longest wavelength band of absorption or emission, an indicator of aggregation; d The % remaining of a 1 μM solution in PBS after several hours of absorption and fluorescence studies. e The % remaining of a, 1 μM solution in PBS allowed to stand in the dark at 4 °C for 48 h (as determined by absorption intensity of Qy band.