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. 2017 Jun 16;22(6):1003. doi: 10.3390/molecules22061003

Figure 1.

Figure 1

Chromatogramm of the HPLC-DAD analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: Phenomenex Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.