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. 2017 Jun 16;22(6):1003. doi: 10.3390/molecules22061003

Figure 4.

Figure 4

Effect of Doronicum austriacum DCM root extract on nitrite release, index of NO production (A,D), measured by Griess reaction, iNOS expression, evaluated by cytofluorimetry (B,E), and on ROS production (C,F), measured by DCHF assay, in J774A.1 macrophages (A–C) and C6 cells (D–F). Data are expressed as mean ± S.E.M.; *** denotes p < 0.001 vs. LPS (1 μg/mL) or LPS + IFN-γ (1 μg/mL; 100 U/mL). L-NAME (1 μM) was used as positive control for inhibition of nitrite release and iNOS expression and inhibited nitrite production with a percentage of 49.00 ± 2.08% and with a percentage of 59.00 ± 0.74% iNOS expression in J774A.1 macrophages. In C6 cells, L-NAME inhibited nitrite release with a percentage of 39.33 ± 4.37% and iNOS expression with a percentage of 46.21 ± 2.65%. N-acetyl cysteine (NAC; 10 mM) was used as positive control for ROS release inhibition, and gave a percentage of inhibition of ROS production of 34.50 ± 0.50% in J774A.1 and of 43.33 ± 1.45% in C6 cells, respectively.