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Synthesized vacuolin-1 analogues inhibited autophagic flux in HeLa cells. (A) A5 (1 µM), not C5 (1 µM), induced the accumulation of yellow LC3B-II puncta in RFP-GFP-LC3B expressing HeLa cells. Scale bar = 10 µm. (B) Treatment of HeLa cells with vacuolin-1 analogues (1 µM) and BAF (100 nM) failed to further increase the accumulation of both LC3B-II and SQSTM1 as compared to either drug alone.
