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. 2017 Jun 8;22(6):954. doi: 10.3390/molecules22060954

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Determination of the time required for the formation of aptamer/antidote (AD) complexes using 10% nondenaturing polyacrylamide gel electrophoresis. Samples were analyzed after 2, 5, 10, 15, and 30 min of incubation in human serum. (A) Analyses of R10-60/R10-60 (AD) FAM complexes; (B) Analyses of NU172/NU172 (AD) FAM complexes. Firstly, FAM-labeled ADs were detected using UV-transilluminator (red colored bands) and then all oligonucleotides were detected using GelRed™ staining (white colored bands). An overlay of FAM and GelRed staining is presented.