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. 2017 Jun 8;22(6):954. doi: 10.3390/molecules22060954

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analyses of specific binding of R10-60 antidote (AD) to the R10-60 aptamer in whole human blood and the formation of aptamer/AD complexes using 10% nondenaturing polyacrylamide gel electrophoresis. Blood was first incubated for 2 min with 5 μg R10-60. Subsequently, 5 μg R10-60 (AD) was added and incubated for 2 min. After isolation of oligonucleotides, samples of 10 μL were analyzed. NS and AD_NS were used as negative controls. Firstly, FAM-labeled ADs were detected using an UV-transilluminator (red colored bands) and then all oligonucleotides were detected using GelRed™ staining (white colored bands). An overlay of FAM and GelRed staining is presented. NS: R10-60 (NS), AD: R10-60 (AD) FAM, AD_NS: R10-60 (AD_NS) FAM.