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. 2017 Jun 8;22(6):951. doi: 10.3390/molecules22060951

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of alisol F and 25-anhydroalisol F on LPS-induced activation of the MAPK signaling pathway. RAW 264.7 cells were pretreated with the different concentrations (0, 3.3, 11 and 33 μM) of alisol F (A) and 25-anhydroalisol F (B) for 2 h, then stimulated with or without LPS (1 μg/mL) for 30 min. The expressions of phospho-ERK1/2, ERK1/2, phospho-p38, p38, phospho-JNK, and JNK proteins were analyzed by Western blot. The data represent the means ± S.E. of three independent experiments. (^## p < 0.001 vs. control group; * p < 0.05; ** p < 0.001 vs. LPS group).

The effect of alisol F and 25-anhydroalisol F on LPS-induced activation of the MAPK signaling pathway. RAW 264.7 cells were pretreated with the different concentrations (0, 3.3, 11 and 33 μM) of alisol F (A) and 25-anhydroalisol F (B) for 2 h, then stimulated with or without LPS (1 μg/mL) for 30 min. The expressions of phospho-ERK1/2, ERK1/2, phospho-p38, p38, phospho-JNK, and JNK proteins were analyzed by Western blot. The data represent the means ± S.E. of three independent experiments. (^## p < 0.001 vs. control group; * p < 0.05; ** p < 0.001 vs. LPS group).