Skip to main content
. 2018 Sep 10;7:e36158. doi: 10.7554/eLife.36158

Figure 5. DMF suppresses TH17 differentiation by augmenting ROS generation.

(A) Naive CD4+ T cells from C57BL/6 mice were differentiated under TH17 or iTreg-inducing conditions with or without H2O2 (1 µM) for 5 days, followed by intracellular staining of IL-17 and Foxp3. (B) Cell proliferation of active CD4+ T cells (72 hr) with or without H2O2 (1 µM) was determined as CFSE dilution. (C–D) Naive CD4+ T cells from C57BL/6 mice were differentiated under TH17-inducing conditions with indicated dose of DMF for 5 days, followed by intracellular staining of IL-17 (C) and ROS (D). (E) Naive CD4+ T cells from C57BL/6 mice were differentiated under TH17-inducing conditions with indicated treatment for 5 days, followed by intracellular staining of IL-17. Data in Figure 5 are representative of two-three independent experiments. Data represent the mean ±S.D.

Figure 5—source data 1. Source data for D.
elife-36158-fig5-data1.xlsx (10.1KB, xlsx)
DOI: 10.7554/eLife.36158.019

Figure 5.

Figure 5—figure supplement 1. DMF suppresses TH17 differentiation through augmenting ROS generation.

Figure 5—figure supplement 1.

(A) Cell proliferation of active CD4 +T cells (72 hr) with indicated treatments was determined as CFSE dilution (B). Naive CD4 +T cells from C57BL/6 mice were differentiated with 5 and 20 µM of DMF under iTreg cell -inducing conditions for 5 days, followed by intracellular staining of Foxp3.