Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) |
C57BL/6 (B6) mice | Taconic | ||
| Strain, strain background (Mus musculus) |
C3H/HeN | Envigo | ||
| Genetic reagent (Mus musculus) |
CD4-Cre
Gclcflox/flox |
PMID:23226398 | ||
| Genetic reagent (Mus musculus) |
Gclm-KO | PMID:12384496 | ||
| Genetic reagent (Mus musculus) |
ROSA26CreERT2 | RRID: IMSR_JAX:008463 |
The Jackson Laboratory |
|
| Genetic reagent (Mus musculus) |
GSR-KO | PMID: 10218442 | ||
| Antibody | Mouse anti- CD3 mAb |
Cat. #:BE0001-1, RRID:AB_1107634 |
BioXcell | |
| Antibody | Mouse anti- CD28 mAb |
Cat. #BE0015-1 RRID:AB_1107624 |
BioXcell | |
| Antibody | Mouse anti -IL2 mAb |
Cat.#BE0043 RRID::AB_1107702 |
BioXcell | |
| Antibody | Mouse anti- IL4 mAb |
Cat. #BE0045 RRID:AB_1107707 |
BioXcell | |
| Antibody | Mouse anti- IFNγ mAb |
Cat. #BE0055 RRID:AB_1107694 |
BioXcell | |
| Antibody | Anti mouse CD4-FITC |
Cat. #11–0042 RRID:AB_464897 |
eBioscience | (1:200) |
| Antibody | Anti mouse CD4-APC |
Cat. #17-0041-81 RRID:AB_469319 |
eBioscience | (1:200) |
| Antibody | Anti mouse CD8-APC-Cy7 |
Cat. #100714 RRID:AB_312753 |
Biolegend | (1:200) |
| Antibody | Anti mouse Foxp3-APC |
Cat. # RRID:AB_469456 |
eBioscience | (1:200) |
| Antibody | Anti mouse IL-17A-PECy7 |
Cat. #25-7177-82 RRID:AB_10732356 |
eBioscience | (1:200) |
| Antibody | Anti GCLC antibody (rabbit monoclonal) |
Cat. #ab190685 RRID:AB_10975474 |
Abcam | WB (1:1000) |
| Antibody | Anti GCLM antibody (rabbit monoclonal) |
Cat. #ab124827 RRID:AB_10975474 |
Abcam | WB (1:1000) |
| Antibody | anti-mouse CD25 -PE |
Cat. #101904 RRID:AB_312847 |
Biolegend | (1:200) |
| Antibody | anti-mouse CD69-PECy7 |
Cat. #552879 RRID:AB_394508 |
BD Bioscience | (1:200) |
| Antibody | Anti mouse monoclonal CD3 |
Cat. #sc-101442 RRID:AB_1120355 |
Santa Cruz | IHC (1:50) |
| Antibody | Anti mouse monoclonal galectin-3 |
Cat. #sc-32790, RRID:AB_627657 |
Santa Cruz | IHC (1:50) |
| Peptide, recombinant protein |
MOG35-55 peptide | synthesized and HPLC-purified |
St. Jude Hartwell Center for Biotechnology |
|
| Peptide, recombinant protein |
Recombinant mouse IL-6 |
216–16 | Peprotech | |
| Peptide, recombinant protein |
Recombinant human TGFb |
100–21 c | Peprotech | |
| Peptide, recombinant protein |
Recombinant human or mouse IL-2 |
200–02 | Peprotech | |
| Commercial assay or kit |
Foxp3/Transcription Factor Staining Buffer Set |
00-5523-00 | e-Bioscience | |
| Commercial assay or kit |
Naive CD4 + T cell isolation kit,mouse |
5160725186 | Miltenyi Biotec | |
| Commercial assay or kit |
CD45R(B220) microbeads, mouse |
5150309030 | Miltenyi Biotec | |
| Commercial assay or kit |
ABC kit | PK-7200 | Vector laboratories |
|
| Commercial assay or kit |
MojoSort Mouse naive CD4 T Cell Isolation Kit |
480031 | Biolegend | |
| Chemical compound, drug |
Diethly Fumerate | Sigma Aldrich | D95654 | |
| Chemical compound, drug |
N-Acetyl-L-cysteine | Sigma-Aldrich | A7250 | |
| Chemical compound, drug |
Tamofixen | Sigma-Aldrich | T5648 | |
| Chemical compound, drug |
4-hydroxytamoxifen | Sigma | H7904 | |
| Chemical compound, drug |
Dimethy a-keto glutarate/aKG |
Sigma-Aldrich | 34963–1 | |
| Chemical compound, drug |
Hypoxathine | Sigma-Aldrich | H9377 | |
| Chemical compound, drug |
Thymidine | Sigma | T9250 | |
| Chemical compound, drug |
H2O2 | Sigma-Aldrich | 7722-84-1 | |
| Chemical compound, drug |
carboxyfluorescein diacetate succinimidyl ester(CFSE) |
Invitrogen | C1157 | |
| Chemical compound, drug |
DM-H2DCFDA | Invitrogen | C6827 | |
| Chemical compound, drug |
DAB | Vector Laboratories |
SK-4100 | |
| Chemical compound, drug |
Monobromobimane | Invitrogen | M1378 | |
| Chemical compound, drug |
7-amino- actinomycin D(7AAD) |
Biolegend | 420404 | |
| Chemical compound, drug |
Pertussis toxin | 181 | List Biological Laboratories |
|
| Chemical compound, drug |
Mycobacterium tuberculosum |
231141 | Difco | |
| Chemical compound, drug |
Incomplete Freund’s Adjuvant |
263910 | Difco | |
| Chemical compound, drug |
[U-14C]-glutamine | MC 1124 | Moravek | |
| Chemical compound, drug |
[2–14C]-pyruvate | ARC 0222 | American Radiolabeled Chemicals |
|
| Chemical compound, drug |
Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) |
00-4975-93 | eBioscience | |
| Chemical compound, drug |
Iscove's Modified Dulbecco's Media - Glucose free conditional medium |
ME17058P1 | Thermo Fisher Scientific |
|
| Chemical compound, drug |
Iscove's Modified Dulbecco's Media - without L-glutamine |
12–726 f | Lonza | |
| Chemical compound, drug |
RPMI 1640 Medium, No Glucose |
11-879-020 | Gibco | |
| Chemical compound, drug |
Hyclone RPMI 1640 Medium, no glutamine |
sh30096.10 | Thermo Fisher Scientific |
|
| Chemical compound, drug |
U-13C6-glutamine | CNLM-1275–0.1 | Cambridge Isotope Lab |
|
| Chemical compound, drug |
6-Diazo-5-oxo-L -norleucine |
D2141-5MG | Sigma-aldrich | |
| Chemical compound, drug |
Bis-2- (5-phenylacetamido -1,3,4-thiadiazol-2-yl) ethyl sulfide (BPTES) |
SML0601 | Sigma-aldrich | |
| Chemical compound, drug |
CB-839 | 22038 | Cayman | |
| Software, algorithm |
Graphpad Prism | RRID:SCR_002798 | ||
| Software, algorithm |
FlowJo | RRID:SCR_008520 |