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. Author manuscript; available in PMC: 2018 Sep 24.
Published in final edited form as: Biotechniques. 2018 Aug;65(2):71–77. doi: 10.2144/btn-2018-0042

Table 5.

Chemiluminescence Gd-IgA1 assay using IgA1 secreted by immortalized IgA1-producing cells.

Cell line Gd-lgA1 (U) / (RLU)
50 ng IgA 25 ng IgA 12.5 ng IgA 6.25 ng IgA 3.125 ng IgA
1 6.00 (U) / (3496) 2.72 (U) / (1445) 2.73 (U) / (1449) –/(578) 2.06 (U) / (1031)
2 10.03 (U) / (6016) 4.00 (U) / (2244) 2.81 (U) / (1497) –/(273) –/(289)
3 4.48 (U) / (2544) 2.42 (U) / (1254) –/(453) –/(335) –/(227)
4 3.99 (U) / (2239) 1.83 (U) / (886) 2.38 (U) / (1231) –/(302) –/(249)
5 7.37 (U) / (4354) 3.13 (U) / (1701) 2.85(U) / (1526) –/(623) –/(301)
6 4.18 (U) / (2355) 1.85 (U) / (901) 1.11 (U) / (436) –/(352) –/(562)

Gd-IgA1 detection over a range of IgA1 amounts using the chemiluminescence assay. ‘–’ denotes values below limit of detection for this assay.

Gd-IgA1: Galactose-deficient IgA1; RLU: Relative light units.