Fig. 5.

PTH differentially regulated β-catenin expression in bones of WT and Fgf2KO mice. Three-month old female mice were treated with PTH (20 μg/kg body weight) or vehicle for 8 h. Left tibia was harvest for RNA extraction and right tibia was harvest for protein extraction. (A) β-Catenin mRNA expression by qPCR, n = 6–11 mice/group. (B) Active vs. total β-catenin protein expression by Western blot and (C) quantification, n = 3 mice/group. (D) Inactive vs. total GSK3β protein expression by Western blot and (E) quantification, n = 3 mice/group. Data presented are Mean ± SE. *: WT-Veh vs. Fgf2KO-Veh p < 0.05; #: compared with corresponding Veh p < 0.05 by ANOVA.