FIG. 2.

Systemic administration of a TLR-2 activator increases laser-induced CNV area. (A) Bar graph and (B) Dot plot of area of CNV ± SEM from an experiment evaluating the area of CNV. Mouse eyes were lasered on day 0, PAM injections were administered to cohorts of mice on different days in relation to the laser application, and CNV area was measured at day 7. Number above the bar is the percentage change relative to the average area of CNV in PBS treated mice. PAM injections increased CNV area with the largest effect observed in mice injected 2 days after laser. CNV area was reduced in mice administered doses of a VEGF Ab, 4G3, at 3 mg/kg i.p. on day 0, 2, and 4. n = 39–58 data points for each group. Statistical analysis performed with an ANOVA with a Dunnett's post hoc test with the PBS treated group as the comparator. P > 0.05. ANOVA, analysis of variance; PBS, phosphate buffered saline; ns, not significant; TLR-2, toll-like receptor-2; VEGF, vascular endothelial growth factor.