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. 2018 Sep 24;13(9):e0201560. doi: 10.1371/journal.pone.0201560

Fig 1. CXCL1, CXCL2 and CCL2 binding to mGEnC-1 is mediated by heparan sulfate (HS).

Fig 1

Serial dilutions of mouse recombinant chemokines CXCL1, CXCL2 and CCL2 were added to TNF-α-activated mouse glomerular endothelial cells (mGEnC-1) and binding was detected using ELISA (A). CXCL1, CXCL2 and CCL2 binding to mGEnC-1 was evaluated with and without TNF-α activation The untreated condition is set at 100% (B). TNF-α-activated mGEnC-1 monolayers were treated with a cocktail of heparinases I, II and III, and binding of CXCL1, CXCL2 and CCL2 was analyzed by flow cytometry. The untreated condition is set at 100% (C). * P < 0.05 vs Untreated, *** P < 0.001 vs Untreated. (OD 450 nm, optical density at 450 nm).