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. 2018 Sep 24;13(9):e0201560. doi: 10.1371/journal.pone.0201560

Fig 3. Chemokine binding to mGEnC-1 is differentially inhibited by mGEnC-1 glycocalyx, HSBK or enoxaparin.

Fig 3

Mouse recombinant chemokines CXCL1 (A), CXCL2 (B) and CCL2 (C) were pre-incubated with serial dilutions (all ranging from 0 to 50 μg/ml) of mGEnC-1-derived glycocalyx, heparan sulfate from bovine kidney (HSBK) or highly sulfated low molecular weight heparin enoxaparin, and incubated with TNF-α-activated mGEnC-1 in ELISA. Results are given as percentage inhibition of mGEnC-1 binding of the chemokines pre-incubated with GAGs compared to chemokine binding in absence of competing GAGs.