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. 2018 Sep 24;13(9):e0201560. doi: 10.1371/journal.pone.0201560

Fig 4. CXCL1, CXCL2 and CCL2 binding to HS on mGEnC-1 is differentially inhibited by anti-HS antibodies that recognize specific HS domains.

Fig 4

mGEnC-1 were pre-incubated with single-chain variable fragment (scFv) anti-HS antibodies that recognize specific domains in HS chains before incubation with CXCL1, CXCL2 and CCL2. Differences in binding signals indicate the variable presence of specific HS domains recognized by the different antibodies in the mGEnC-1 glycocalyx (A). Competition results are depicted as percentage inhibition of chemokine binding to mGEnC-1 compared to binding to untreated cells. CXCL1 (B), CXCL2 (C) and CCL2 (D) binding to mGEnC-1 was differentially inhibited by pre-incubation with the specific scFv anti-HS antibodies. * P < 0.05 vs control, ** P < 0.01 vs control, *** P < 0.001 vs control.