Skip to main content
. Author manuscript; available in PMC: 2018 Sep 24.
Published in final edited form as: Cell Syst. 2018 May 16;6(5):555–568.e7. doi: 10.1016/j.cels.2018.04.011

Figure 4. Fitness correlation mapping identifies biochemically distinct modules of mammalian SWI/SNF complexes.

Figure 4

(A) Schematic depicting subunits of the mammalian SWI/SNF family of ATP-dependent chromatin remodeling complexes.

(B) Fitness correlation network (from RNAi dataset) between mSWI/SNF subunits resolves three functional modules: core BAF (SMARCA4, ARID1A, SMARCB1 and SMARCE1), PBAF (PBRM1, ARID2, BRD7, PHF10) and a novel functional module that contains two previously characterized subunits (SMARCD1, BRD9) and one putative subunit (GLTSCR1).

(C) Hierarchical clustering performed on fitness profile correlations from the RNAi dataset groups subunits into distinct modules.

(D) Density sedimentation experiments using 10-30% glycerol gradients performed on nuclear extracts from CCRF cells links two functional modules to known complexes, BAF (blue bar) and PBAF (red bar), and one to a novel assembly of distinct size and composition (green bar).

(E) Rare cancers characterized by mSWI/SNF perturbations exhibit mutually exclusive loss of one of the BAF core module genes or paralog families (containing SMARCA4, ARID1A, SMARCB1, SMARCE1). SCCOHT = small cell carcinoma of the ovary, hypercalcemic type. In addition, specific intellectual disability syndromes are caused by heterozygous mutations in BAF core module genes. i