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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Dev Biol. 2018 Jul 5;441(1):191–203. doi: 10.1016/j.ydbio.2018.07.003

Figure 5. Constitutive activation of Hh signaling in palatal epithelium upregulates cell adhesion-associated genes and epithelial progenitor cell-associated genes in the MEE.

Figure 5.

(A-F) RNAscope in situ hybridization of Dsg3, Itga3 and Lama5 (red dots, indicated by arrows) in frontal sections of E14.5 control and K14-Cre;R26SmoM2 palatal shelves. (G) qPCR analysis of Dsg3, Itga3 and Lama5 in E14.5 control and K14-Cre;R26SmoM2 palatal shelves. Values are expressed relative to control. *, p<0.05. (H-I) RNAscope in situ hybridization analysis of Mmp13 (red dots, indicated by arrow) in frontal sections of E14.5 control and K14-Cre;R26SmoM2 palatal shelves. Arrowhead indicates absence of Mmp13 expression. (J-K) Immunofluorescence of ΔNp63 (p63, red) and Sox2 (green) in frontal sections of E14.5 control and K14-Cre;R26SmoM2 palatal shelves. Boxed areas in (J) and (K) are shown magnified in the top right corners with red (p63), green (Sox2), blue (DAPI) and merged channels. Arrows indicate co-expression of ΔNp63 and Sox2. (L-M) RNAscope in situ hybridization analysis of Lgr5 (red dots, indicated by arrows) in frontal sections of E14.5 control and K14-Cre;R26SmoM2 palatal shelves. Black dotted lines indicate the location of the MEE. Scale bars: 50 μm.