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. Author manuscript; available in PMC: 2019 Nov 22.
Published in final edited form as: Anal Chim Acta. 2018 May 4;1032:1–17. doi: 10.1016/j.aca.2018.05.011

Fig. 11. Schematic diagram of the detection procedures for simultaneous and combined detection of multiple tumor markers (TMs) for prostate cancer (PC by suspension array.

Fig. 11.

(A) The carboxylated blank red and orange fluorescent beads were activated.

(B) The free TM target was coupled onto activated carboxylated beads by EDC/NHS to prepare bead probe set as the beadeTM compound.

(C)The TM on the bead and the free TM were allowed to compete for their corresponding mAb in solutions; then, the beadeTMemAb compound was formed.

(D)Sec-Ab- biotin was added to capture them Ab in solutions and to result in the beadeTMemAbesec-Ab-biotin compound.

(E) Streptavidin-R-phycoerythrin (SA-PE) was added into the solutions to couple with the compound by biotin-avidin interaction. The beadeTMemAbesec-Ab-biotineSA-PE compound was ready for laser beaming. The red laser (Ex1) was used to excite the red and orange fluorescent dyes within the beads to recognize their unique coded numbers. Simultaneously, a green laser (Ex2) was used to excite SA-PE that combined on the bead surface. The available fluorescent signalsdMFIs that only combined on the beads were recorded and analyzed by xPONENT™ 3.0 software. Reprinted from [96] With permission. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)